The Children's Rights Alliance is calling for the creches in last night's documentary to be closed down.
An RTÉ Investigates report showed rough handling of children, and babies restrained for long periods.
Undercover reporters looked into the standards of care in the Hyde and Seek Childcare creche chain in Dublin - on foot of concerns raised by several families.
The company has four creches across Dublin.
Last night's documentary showed how one of the creches - in Glasnevin - was unregistered for some 14 months.
It also highlighted repeated breaches of regulations.
Up to 20 children were left in one worker's care for an hour at a time in one creche.
The Hyde and Seek group says the documentary raises real issues, but it says some of them have already been addressed.
It says all its creches are now fully registered and regulated by Tusla - and it encourages parents to contact it with any concerns.
Tusla says the documentary raises serious concerns about the quality of care within these crèches.
The Children's Rights Alliance says action must be taken. It says they should face sanctions, cuts to public funding, and the facilities should be closed.
